Myocardial Work Lab

Directions:  Click and download Excel File for trained and untrained subject to your disk.  You do not have to use your lab sections data.

 



Tuesday and Wednesday Labs

Wed 9 am trained & untrained
Wed 11:30  am trained & untrained
  Wed 12:30 trained and untrained
 
  Click here for sample graph (from Lindsey)
 

 

ESTIMATION OF MYOCARDIAL WORK DURING EXERCISE

INTRODUCTION
Exercising muscles require greater blood flow than resting muscles. One of the important factors in bringing about greater blood flow is the increase in the arterial systolic pressure.  Since diastolic pressure in the normal young individual changes very little with exercise, the change in mean arterial pressure during exercise depends primarily upon the systolic blood pressure which is influenced by the cardiac output and the decreased peripheral resistance caused by vasodilation of the micro-circulation (capillaries).  In comparing both rhythmic and static work in the muscles, a greater perfusion (arterial) pressure is needed to provide blood flow in the muscle involved in a static contraction.  This added pressure is somewhat proportional to the intensity of the contraction.

It has also been shown that myocardial oxygen consumption ( MVO2) is highly correlated to the product of heart rate and systolic blood pressure.  This number, called the Rate-Pressure Product (RPP) or Double Product, can be used effectively in the evaluation of a cardiac patient's response to exercise since the magnitude of this value is also related to the onset of angina pectoris caused by myocardial ischemia (Robinson, 1967).  Of added interest is the effect of static exercise on this index.  It has been shown that static contractions can al so increase the double product disproportionately and thereby increase the demands on the heart.  The extent of this increase is dependent on the relative force of the contraction as well as the duration of the event, but not to the absolute tension produced or to the bulk (mass) of the muscle activated.

PURPOSE
The purpose of this lab is to measure the heart rate and blood pressure response to static and dynamic exercise and relate these changes to the stress upon the heart as indicated by the Rate-Pressure Product.

MATERIALS
 1.  bicycle ergometer
 2.  stethescope and sphygomanometer
 3.  hand grip dynamometer
 4.  data sheet
 5.  work clock

PROCEDURE
Data will be collected as follows on a trained and sedentary individual.

1.  Adjust the seat of the ergometer to the appropriate height.  Determine 35% and 70% of MVC using the handgrip dynamometer.  Attach the BP cuff to the upper n on-dominant arm.

 2.  Obtain resting HR, Systolic and Diastolic BP.

3. Have the subject ride at a moderate workload (1-1.5 Kp/min for females and 2-2.5 Kp/min for males) at a rate of 50 rpm for a period of six (6) minutes.  Record HR and BP (systolic only) on alternate minutes (ie: 2, 4, and 6 min).

4.  Without interruption, at the beginning of the seventh minute have the subject remove the foot from the pedal on the same side as the BP is being taken and rest it on the foot support so that he/she now pedals one-legged at the same workload.  Record HR and BP each minute for two (2) minutes of one-legged pedaling.

5.  Now, without interruption, have the subject return to two-legged pedaling for three (3) minutes.  Record HR and BP for each minute.

 6.  Next, without interruption, have the subject hold a 35% maximal voluntary contraction (MVC) on dynamometer the handgrip with the dominant hand for one (1) minute.  Record the HR and BP at 30 sec and one minute of this stage.

7.  Release the grip and continue to ride for another 4 minutes while maintaining the same workload.  Record HR and BP at 2 and 4 min.

8.  Next, repeat the static contraction of step #6 using the 70% MVC on the dominant hand for one (1) min.  Record the HR and BP at 30 sec and one minute of this stage.

9.  Finally, have the subject pedal at the same workload for six (6) additional min.  Record HR and BP at 2, 4, & 6 min.

CALCULATIONS
Compute the RPP and MVO2 for each minute according to the following formulas:

  RPP = HR x SBP x 0.01

  MVO2 ml/100gLV/min = (0.16 x RPP) - 6.0
 

RESULTS (GRAPHS)

You have six (6) graphs to complete.  The x-axis on all 6 graphs will be time (in minutes or seconds).  I suggest that you make x-y scatter plots, save the graphs and then change the "data source" to the new numbers you need on your spread sheet  It will save you a bunch of time.  Also, remember units and remember that you need to indicate on the graphs the different conditions (e.g. 1-leg cycle etc.).  List of graphs:

1.  MVO2 vs time for your groups subject
2.  HR vs. time for your subject
3.  SBP vs. time for your subject
4.  MVO2 of trained untrained vs. time
5.  HR of trained and untrained vs. time
6.  SBP of trained and untrained vs. time
 

RESUL TS (DATA TABLE)

Complete the Table as per instructed in class; typed in Excel
 

DISCUSSION (QUESTIONS)


1.  Review the data which shows the response of the MVO2 to the two-legged dynamic exercise, the one-legged dynamic exercise, the 35% MVC imposed on the dynamic exercise, and the 70% MVC imposed on the dynamic exercise.  What is the effect of an isometric contraction by even a small muscle mass upon the work of the heart?  Is the re a difference in the work of the heart between the trained vs. untrained subjects.  Give an explanation for your answers.

2.  On the assumption that the same work was being performed with one leg as with two, what effect does the amount of muscle mass recruited to perform a task have upon the work of the heart when the total work accomplished by the individual is the same?

3.  Describe in detail the procedures to measure blood pressure.  If you use our text book to answer the question, make sure you provide a reference.
 

DATA TABLES


CARDIOVASCULAR REPONSE TO ISOMETRIC EXERCISE IN AN ENDURANCE-TRAINED SUBJECT DATASHEET

SUBJECT _______________________________     DATE _________________

WL ___ Kp/min  HR b/min SBP mm Hg   RPP     MVO2 ml/100gLV/min

Rest  _________ ___________ ________ ____________

Exercise
 2 min _________ ____________ ________ ___________ _

 4 min _________  ____________ ________ _____________

 6 min _________ ____________ ________ _____________

One-legged
 1 min _________ ____________ _________ ______________

 2 min _________ _____________ _________ ______________

two-legged
 1 min _________ _____________ _________ ______________

 2 min _________ _____________ _________ ______________

 3 min _________ _____________ _________ ______________

35% MVC
 3 0 sec _________ _____________ _________ ______________

 1 min _________ _____________ _________ ______________

Exercise
 2 min _________ _____________ _________ _______________

 4 min _________ _____________ _________ _______________

70% MVC
 30 sec _________ _____________ _________ _______________

 1 min _________ _____________ _________ _______________

Exercise
 1 min _________ _____________ _________ ________________

 2 min ___ ______ _____________ _________ ________________

 3 min _________ _____________ _________ ________________
 
 

CARDIOVASCULAR RESPONSE TO ISOMETRIC EXERCISE
IN A SEDENTARY SUBJECT DATASHEET

SUBJECT _______________________________     DATE _________________

WL ___ Kp/min  HR b/min SBP mm Hg   RPP     MVO2 ml/100gLV/min

Rest  _________ ___________ ________ ____________

Exercise
 2 min __ _______ ____________ ________ ____________

 4 min _________  ____________ ________ _____________

 6 min _________ ____________ ________ _____________

One-legged
 1 min _________ ____________ _________ ______________

 2 min _________ _____________ _________ ______________

two-legged
 1 min _________ _____________ _________ ______________

 2 min _________ _____________ _________ ______________

 3 min _________ _____________ ________ _ ______________

35% MVC
 30 sec _________ _____________ _________ ______________

 1 min _________ _____________ _________ ______________

Exercise
 2 min _________ _____________ _________ _______________

 4 min _________ _____________ _________ _______________

70% MVC
 30 sec _________ _____________ _________ _______________

 1 min _________ _____________ _________ _______________

Exercise
 2 min _________ _____________ _____ ____ ________________

 4 min _________ _____________ _________ ________________

 6 min _________ _____________ _________ ________________

Estimation of Myocardial Work
Table 1: Estimated work of the heart during cycling in a trained and untrained subject by rate-pressure-product.
________________________________________________________________________
     Untrained RPP Trained RPP
________________________________________________________________ ________
Two-leg (6 min)
One-leg (8 min)
35% MVC
70% MVC
Two-leg (20 min)
________________________________________________________________________