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Extra Credit Data.  Click and save data as new Excel File. You can choose any groups data
  Wed. 8 am lab group 1 Wed. 8 am lab group 2
  Wed. 10 am lab  group 1 Wed. 10 am lab  group 2
  Wed. 12 lab group 1 Wed. 12 lab group 2


As we have discussed in previous labs and in lecture, one of the most widely used methods of work quantification in both clinical and research applications of exercise physiology involves the measurement of oxygen consumption. Maximal oxygen uptake (VO2max) is widely accepted as the best index of cardiorespiratory endurance capacity, while submaximal and resting oxygen uptake measurements provide useful information relative to metabolic efficiency.

Maximum oxygen uptake can be measured using any number of experimental protocols, ergometric devices, and gas analysis procedures. Regardless of the testing and analysis method utilized, however, at least three specific criteria must be met to confidently call a VO2max test a true "max test". These are as follows:

1. a peak or plateau in oxygen uptake with continued increases in workload
2. a peak or plateau in heart rate with continued increases in workload
3. a RER value of >1.10
*4. a blood lactate level of >14 mmol (this criteria is not used as extensively as the others)
5.  RPE = or > 18

VO2max measurements are used for several reasons including:

1. to discriminate between those people with high endurance ability from those with only ordinary or minimal capacity
2. to evaluate the effects of different training procedures on endurance ability
3. to measure the metabolic cost of various work tasks under normal or novel environmental conditions (heat, altitude, cold) in order to develop more effective ways of working and eliminating fatigue
4. as a diagnostic tool in the detection of impaired cardiorespiratory function or muscle metabolic diseases
When measuring the oxygen uptake of an exercising subject two parameters which must be measured are 1) the minute ventilation--the amount of air the subject inspires or expires per minute, and 2) the oxygen and carbon dioxide concentration of the expired air.

In determining oxygen consumption, we are interested in knowing how much oxygen has been removed from the inspired air. Because the composition of inspired air remains relatively constant, it is possible to determine how much oxygen has been removed from the inspired air by measuring the amount and composition of the expired air. When this is done, the expired air contains more carbon dioxide, less oxygen, and more nitrogen. It should be noted, however, that nitrogen is inert in terms of metabolism; any change in its concentration in expired air reflects the fact that the number of oxygen molecules removed from the inspired air are not replaced by the same number of carbon dioxide molecules produced in metabolism. This results in the volume of expired air ( VE, STPD) being unequal to the inspired volume (VI, STPD). For example, if the RQ is less than 1.00 (ie: less CO2 produced to O2 consumed), and 3 liters of air are inspired, less than 3 liters of air will be expired. In this case, the nitrogen concentration is higher in the expired air than in the inspired air. This is not to say that nitrogen has been produced, only that nitrogen molecules now represent a larger percentage of the VE compared to the VI (see McArdle, Katch, & Katch: pp 799-803). Because nitrogen is an inert gas, its concentration can also be used to determine the volume of air ventilated through a derivation called the Haldane Transformation.


The purpose of this laboratory is to introduce the manual method of gas collection/analysis and to measure resting and maximum oxygen consumption.


1. treadmill or bicycle ergometer
2. work clock
3. heart rate monitor
4. 1-way breathing valve; low resistance, large-bore tubing; Hans Rudolph 3-way valve
5. electronic CO2 and O2 analyzers
6. thermometer, barometer
7. meterological balloons or computer system
8. Rayfield ventilation (gas) meter


1. choose two men and two women to perform measures of resting and exercise metabolism.

2. choose a trained and untrained subject to perform a maximal treadmill test; attach a heartrate monitor; adjust headset and mouthpiece; adjust noseclip

3. set up gas collection bags using the 100 liter meterological balloons, the 3-way Hans Rudolph valve, and the Rayfield gas meter

4. measure room temperature and barometric pressure; using Tables C-1 & C-2 on pages 797 & 798 in your text, determine the vapor pressure (PH2O) and the STPD correction factor respectively; place in the appropriate place on the datasheet

5. assign group members to perform the following tasks:

a. monitor workload and clock

b. monitor heartrate

c. operate three-way valve

d. attach and replace ventilatory bags

e. analyze gas composition

f. determine gas volumes (two technicians)

g. record data (two technicians)

6. have subject straddle the treadmill belt; start treadmill; begin data collection after subject has warmed up properly (approx 3-5 min of low intensity exercise)

7. follow the treadmill protocol as determined prior to the test

8. Gas Collection and Analysis

a. once data collection has begun, make sure that all the subject's expired air is collected in the balloons; one balloon will be collected during for a minute after 5 min of rest.  A small sample of gas will be drawn from the balloon into the oxygen and carbon dioxide analyzers (timed 1 min sample); record the gas concentrations on the data sheet in the appropriate place.  The volume of the gas will be determined by driving the contents of the balloon through the Rayfield gas meter; record the volume and temperature of the gas in the appropriate place on the datasheet

Extra Credit Graphs and Questions

Make the following 4 Graphs (make sure you have correct units, We will not accept extra credit graphs unless they are correct). For 4 extra credit lab points!  These graphs are due in lab in ONE WEEK!